TABLE 1.
Summary of data collection and refinement statistics
| Parametera | Result |
|
|---|---|---|
| CLSV | RCNMV | |
| Data collection | ||
| No. of micrographs | 1,246 | 620 |
| Defocus range (μm) | 0.8–2.5 | 0.8–2.5 |
| Dose rate (e/pix/s) | ∼7 | ∼7 |
| Total dose (e/Å2) | ∼50 | ∼50 |
| No. of particles picked | 37,556 | 100,007 |
| No. of particles used | 5,908 | 3,966 |
| Refinement statistics | ||
| PDB ID | 6MRL | 6MRM |
| EMDB ID | 9204 | 9205 |
| Resolution (Å) | 3.2 | 2.9 |
| Rmodel (%) | 34 | 36 |
| Correlation coefficient (%) | 85 | 82 |
| Ramachandran favored (%) | 85 | 82 |
| Ramachandran outlier (%) | 0 | 1.2 |
| Rotamer outlier (%) | 2 | 0.3 |
| C-β outlier | 2 | 3 |
| Bond length RMSD (Å) | 0.01 | 0.01 |
| Bond angle RMSD (°) | 1.5 | 1.7 |
| EMringer | 4.5 | 3.2 |
| ClashScore | 12.1 | 6.7 |
| MolProbity | 2.2 | 2.1 |
a
EMDB, Electron Microscopy Data Bank; ID, identifier; pix, pixel; RMSD, root mean square deviation.