FIG 1.

Effect of CTCF or Rad21 depletion on KSHV virion production. (A) KSHV-infected iSLK cells were transfected with either control siRNA (NC Si), CTCF-specific siRNA (CTCF Si) or Rad21-specific siRNA (Rad21 Si). KSHV replication was induced by doxycycline treatment (+D) or mock induced (−D). Supernatants from induced cells were used to infect 293T cells. Virus passage was quantitated by flow cytometry of GFP-positive 293T cells. Each transfection/induction was performed in triplicate, and three replicate infections were performed with each supernatant. Error bars denote standard errors of the means (SEM) for all experiments. (B) Western blot showing the efficacy of CTCF and Rad21 KD with actin as a loading control. (C) Cells were counted after each KD with vital dye staining. A minimum of 200 cells per sample were counted.