FIG 7.

Effect of depletion of CTCF or Rad21 on cellular interferon-regulated gene expression. (A) Depletion of CTCF and/or Rad21 in iSLK/Bac16 cells followed by KSHV lytic replication induction was performed. RNA was prepared 48 h after the induction of replication. Relative quantification of mRNA expression (RQ) for each lytic gene was performed by qPCR. Results for Stat2, OAS1, OAS1, OAS2, OAS3, OASL, and IFIT1 are shown. Each transfection was performed in triplicate, and qPCR was performed with three technical replicates per sample. The level of expression of each RNA was normalized to the level of expression in uninduced cells (NC Si, negative-control siRNAs). (B) Uninfected iSLK cells were transfected with either negative-control siRNA, CTCF siRNA, or Rad21 siRNA. RNA was harvested, and qPCR was performed for ISGs as described above for panel A. (C) Efficiency of CTCF and Rad21 KD in panel B measured by Western blotting, with actin as a loading control.