FIG 3.

Neutralization data for IgG and scFv of HIV bNAbs tested against the 45-virus panel. The IC₅₀ values for both IgG and scFv against 7 subtype A, 12 subtype B, and 26 subtype C viruses are shown in micrograms per milliliter. IgG was tested at a starting concentration of 50 μg/ml, whereas scFv started at 250 μg/ml. Fold changes between IgG and scFv are shown. The colors indicate the potency at which the viruses were neutralized, with the warmer colors indicating higher potency. Resistant viruses are colored in light blue. Maximum levels of neutralization for scFv and IgG of <50% but >20% are indicated with an asterisk. Geometric mean titers, fold difference from IgG, and breadth against the 45-virus panel are shown at the bottom of the table. (scFv titers were corrected for molecular weight size difference by multiplying their IC₅₀ by the fold difference in molecular weight between the IgG and the scFv, as noted in Fig. 1A).