TABLE 2.
Primer sequences to create chimeric RBD plasmidsa
| Protein | Original plasmid | Forward primerb | Reverse primerb |
|---|---|---|---|
| MMQ | M41-RBD | gtcgcttccgtgctagca | acaccagrtckccgttcag |
| QX-RBD | ctgaacggmgayctggtgt | ctgcttcatgcgcttaattaa | |
| gtcgcttccgtgctagca | ctgcttcatgcgcttaattaa | ||
| MQM | MQQ | gtcgcttccgtgctagca | acaccagrtckccgttcag |
| M41-RBD | ctgaacggmgayctggtgt | ctgcttcatgcgcttaattaa | |
| gtcgcttccgtgctagca | ctgcttcatgcgcttaattaa | ||
| MQQ | M41-RBD | gtcgcttccgtgctagca | tagcaatgwgtsacgaacactg |
| QX-RBD | cagtgttcgtsacwcattgcta | ctgcttcatgcgcttaattaa | |
| gtcgcttccgtgctagca | ctgcttcatgcgcttaattaa | ||
| QQM | QX-RBD | gtcgcttccgtgctagca | acaccagrtckccgttcag |
| M41-RBD | ctgaacggmgayctggtgt | ctgcttcatgcgcttaattaa | |
| gtcgcttccgtgctagca | ctgcttcatgcgcttaattaa | ||
| QMQ | QMM | gtcgcttccgtgctagca | acaccagrtckccgttcag |
| QX-RBD | ctgaacggmgayctggtgt | ctgcttcatgcgcttaattaa | |
| gtcgcttccgtgctagca | ctgcttcatgcgcttaattaa | ||
| QMM | QX-RBD | gtcgcttccgtgctagca | tagcaatgwgtsacgaacactg |
| M41-RBD | cagtgttcgtsacwcattgcta | ctgcttcatgcgcttaattaa | |
| gtcgcttccgtgctagca | ctgcttcatgcgcttaattaa | ||
| M41-SGS | M41-RBD | atctgatggatgtcccatcacc | ccggacttatagcaatgtgtcacg |
| M41-KIP | M41-RBD | tcctaagaactttctgcgggtgtc | atcttgccggtgatgggacatcc |
| M41-SGS-KIP | M-SGS | tcctaagaactttctgcgggtgtc | atcttgccggtgatgggacatcc |
| QX-Y | QX-RBD | ctgtcccatcaccggcaag | tacccggagctgtagcaatg |
| QX-MLQ | QX-RBD | gcagcgggaccacatcagaatttc | agcatgccggtgatgggacaaga |
| QX-Y-MLQ | Q-Y | gcagcgggaccacatcagaatttc | agcatgccggtgatgggacaaga |
a
R, nt A/G; K, nt G/T; M, nt A/C; Y, nt C/T; W, nt A/T; S, nt C/G.
b
Underlined sequences indicate nucleotides changed to introduce the mutation.