TABLE 4.
qRT-PCR primers and corresponding hydrolysis probes used in this studya
| Target | Forward primer (5′–3′) | Hydrolysis probe (5′–3′) | Reverse primer (5′–3′) |
|---|---|---|---|
| DENV2 New Guinea C | TCGGAGCCGGAGTTTACAAA | 6FAM-ATTCCATACAATGTGGCA-MGB | TCTTAACGTCCGCCCATGAT |
| ZIKV976 | AAATACACATACCAAAACAAAGTGG | 6FAM-CTCAGACCAGCTGAAG-MGB | TCCACTCCCTCTCTGGTCTTG |
| ZIKVH/PF/2013 | AAGTACACATACCAAAACAAAGTGG | 6FAM-CTTAGACCAGCTGAAA-MGB | TCCGCTCCCCCTTTGGTCTTG |
a
MGB, TaqMan minor groove binder (quencher); OQA, Onyx Quencher A; 6FAM, 6-carboxyfluorescein.