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. 2020 Jan 6;94(2):e01132-19. doi: 10.1128/JVI.01132-19

FIG 6.

FIG 6

Tim-3+ cells preferentially upregulate genes that are associated with T cell activation. TGs from acutely infected mice (8 dpi) were removed, processed into a single-cell suspension, and stained for viable cells, CD45, CD8, gB tetramer, Tim-3, and PD-1. CD8+ T cells were gated on gB-CD8+ T cells or Subdom-CD8+ T cells using gB tetramer; Tim-3− PD-1−, Tim-3+ PD-1−, Tim-3+ PD-1+, and Tim-3− PD-1+ cells were sorted, and their transcriptional profile was assessed by using NanoString technology. (A) Genes of interest that averaged a fold change of ≥1.5 over 3 independent replicates between Tim-3+ PD-1− gB-CD8+ T cells and Tim-3− PD-1− gB-CD8+ T cells. (B) Fold change of genes of interest between Tim-3+ PD-1− Subdom-CD8+ T cells and Tim-3− PD-1− Subdom-CD8+ T cells. (C) Fold change of genes of interest between Tim-3+ PD-1− gB-CD8+ T cells and Tim-3+ PD-1− Subdom-CD8+ T cells.