FIG 3.

HPV16 E5 is sufficient to suppress IFN-κ expression. (a) NOKs that were previously hTert immortalized were transduced with retroviruses containing an empty vector (LXSN-Empty), a vector expressing the HPV16 E6 protein alone (LXSN-16E6), or a vector expressing the wild-type HPV16 E5 protein (LXSN-16E5). Following selection and outgrowth, IFNK levels were measured using RT-qPCR. (b) Primary HFKs were transduced with retroviruses expressing either E6/E7 alone or E6/E7 IRES E5. Following selection, the immortalized colonies were pooled, and IFNK levels were measured using RT-qPCR. ***, P < 0.001. The error bars indicate standard error of the mean.