FIG 4.

Suppression of ISGs in HPV16⁺ cells is E5 dependent. (a) Levels of the indicated ISG mRNAs in HFK, HPV16⁺, and E5 Stop cells were measured using RT-qPCR. (b) Immunoblots showing the levels of OAS2 protein (top) and IFIT1 (bottom) in HFK, HPV16, and E5 Stop cells. (c) Levels of the indicated ISG mRNAs in NOKs transduced with empty or E6- or E5-expressing retroviruses were measured by RT-qPCR. (d) Levels of the indicated ISG mRNAs in HFKs immortalized with retroviruses expressing E6/E7 alone or E6/E7 IRES E5 were measured using RT-qPCR. The statistics in panels c and d are relative to HFK. **, P < 0.01; ***, P < 0.001. The error bars indicate standard error of the mean.