FIG 4.

Coimmunoprecipitation of MuV-HN with the F protein. WT and mutant HN proteins were HA tagged, whereas the F protein was FLAG tagged. HEK293 cell transiently expressing HN and F proteins were lysed and immunoprecipitated (IP) with anti-FLAG Ab. The signal intensities of IP and corresponding whole-cell lysate (Input) samples on the membrane as detected with anti-HA Ab were quantified by using the VersaDoc 3000 imager. The ratio of the intensity of the IP sample to that of Input is indicated below.