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. 2019 Dec 23;117(1):285–291. doi: 10.1073/pnas.1911360117

Fig. 3.

Fig. 3.

The effect of USSN on the phosphorylation of Zap70 and LAT in T cells. (A) A representative immunoblot of cultures stimulated for 0.2 to 1 h, showing the effect of 800 μM USSN on the phosphorylation of Zap70 in Jurkat cells. Blots were probed with antibodies to detect pZap70 (at Tyr319, protein ∼70 kDa) and vinculin (protein ∼128 kDa). (B) Percentage of pZap70 band volume compared to the vinculin band control at 0.2 to 1 h. Western blot data represent the means ± SD for 3 independent replicates. (C) Percentage of pZap70 band volume compared to the vinculin band control at 0.2 to 6 h in enriched T cell cultures. Western blot data represent the means ± SD for 6 different donors (*P < 0.05, denotes significance compared to the control, paired t-test). (D) A representative immunoblot of cultures stimulated for 0.2 h, showing the effect of 800 μM USSN on the phosphorylation of LAT in enriched T cells. Blots were probed with antibodies to detect pLAT (at Tyr191, protein ∼37 kDa) and vinculin (protein ∼128 kDa). (E) Percentage of pLAT band volume compared to the vinculin band control at 0.2 h in enriched T cell cultures. Western blot data represent the means ± SD for 6 different donors (*P < 0.05, denotes significance compared to the control, paired t-test).