Fig. 4.

Relationship between localization accuracy and BG complexity. To account for the influence of the SBR, which trivially has an effect on the localization accuracy, the BG complexity metric was normalized by the SBR. For increasing BG complexity, the accuracy of the localization decreases, but in all cases, the BGnet outperforms constant BG subtraction. (A–D) Results from simulated data for the OA PSF, DH PSF, arbitrary PSF, and Tetra6 PSF, respectively. True, BG correction with true BG; predicted, BG correction with the prediction from BGnet; constant, constant BG estimate. Note that the “streaks” visible arise from the discrete SBRs considered. Also note different y-axis scaling in the top and bottom graphs for each PSF. a.u., arbitrary units.