Fig. 4.

AF-miRNAs target structural, signaling, and remodeling components of the ECM. (A and B) Activities of wild-type and mutant (mutated miRNA site) 3’-UTR–luciferase constructs derived from (A) Pdgfa in NIH/3T3 cells transfected with miR-29c and scrambled miRNA mimic and (B) Tgfbr1 in NIH/3T3 cells transfected with let-7g and scrambled miRNA mimic. Let-7g– and miR-29c–transfected samples are colored in the plots according to the luciferase construct schematic. Samples transfected with scrambled miRNA mimic are shown in white (Neg. ctrl). (C and D) Relative expression of putative let-7 target genes associated with fibrosis in stable Lin28a-overexpressing NIH/3T3 cells (C) and putative miR-29c target genes associated with fibrosis in NIH/3T3 cells transfected with miR-29c mimics (D). (A and B) Data are shown as median, first, and third quartiles (“box”) and 95% confidence interval of median (“whiskers”). (C and D) Data are shown as mean and SEM. *p value $\le$ 0.05, **p value $\le$ 0.01, ***p value $\le$ 0.001.