Table 1.
Survival of wild-type M. abscessus ATCC19977, ΔMab_HflX, ΔMab_HflX +phsp-HflX, ΔMab_erm41, and ΔMab_HflX/ΔMab_erm41 in a 2-fold dilution series of antibiotics in Middlebrook 7H9/OADC medium
| Antibiotic | Minimum Inhibitory Concentration (μg/mL) | |||||
| WT Mab | ΔMab_hflX | ΔMab_hflX + phsp-hflX | ΔMab_whiB7 | ΔMab_erm41 | ΔMab_hflX/ΔMab_erm41 | |
| Erythromycin | 2 | 0.25 | 2 | 0.625–0.125 | 0.25 | 0.125 |
| Clarithromycin | 1 | 0.125 | 1 | 0.0625 | 0.125 | 0.0625 |
| Azithromycin | 8 | 2 | 16 | 0.5 | 2 | 1.0 |
| Clindamycin | 200 | 50 | 200 | 12.5 | 25 | 25 |
The minimum concentration of antibiotic required to inhibit 99% of growth after 72 h is shown. Minimum inhibitory concentration values are representative of 3 independent assays.