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Vaccine experimental design and schedule. PBS—phosphate-buffered saline.
| Group | Animals, n | n Euthanized at x Days After 4th Dose | ||
|---|---|---|---|---|
| x = 3 | x = 7 | x = 14 | ||
| PBS | 15 | 5 | 5 | 5 |
| Adjuvant (AFPL1) | 15 | 5 | 5 | 5 |
| Vaccine | 15 | 5 | 5 | 5 |
| Schedule | Route | Volume | ||
| Day 0 | IM/IN | 200 µL (100 µL per leg)/80 µL (40 µL per nare) | ||
| Day 21 | IN | 80 µL (40 µL per nare) | ||
| Day 42 | IN | 80 µL (40 µL per nare) | ||
| Day 56 | IN | 80 µL (40 µL per nare) | ||
