Figure 5.

RNAseq genes associated with NRF2-mediated oxidative stress response via IPA^®. Toxic doses of METH can produce oxidative stress in the rodent brain. In the rat striatum 2 h post METH, IPA^® showed that the upregulation of a number of genes associated with oxidative stress are under the control of NRF2, which is itself upregulated by METH. Among the upstream mediators which can initiate this pathway are drugs, cytokines prostaglandins, and bacterial infections. RNAseq data showed that Il1a, Il1b, and the enzymes which can enhance synthesis of PGE2 are also upregulated by METH and could contribute to initiating the NRF2-mediated oxidative stress response. Red symbols = upregulated genes. Green symbols = downregulated genes. Gene expression data can be found in Table S3.