Table 6.
RNAseq data for top 25 genes enriched in microglia [32]. (U) = unique to microglia vs. oligodentrocyes, astrocytes, and neurons. Bold text = change in expression FDR <0.05 METH vs. Saline treatment. ND = not detected. NS = not significant.
| Symbol | Gene Name | STR Average log2cpm Saline (n = 6), METH (n = 8) | STR Fold Change, FDR 2 h METH vs. Saline | PFC Average log2cpm Saline (n = 4), METH (n = 5) | PFC Fold Change, FDR 2 h METH vs. Saline | ||
|---|---|---|---|---|---|---|---|
| P2ry12 (U) | purinergic receptor P2Y12 | 10.88, 9.77 | −2.01, 5.0 × 10−5 | 10.93, 10.23 | −1.57, 4.5 × 10−5 | ||
| Tmem119 (U) | transmembrane protein 119 | 11.24, 11.04 | −1.19, 0.39 (NS) | 11.28, 11.38 | 1.11, 0.72 (NS) | ||
| Fcrls (Fcrl2) (U) | Fc receptor-like 2 | 10.04, 9.81 | −1.08, 0.83 (NS) | 10.51, 10.44 | −1.11, 0.81 (NS) | ||
| Olfml3 (U) | olfactomedin-like 3 | 1.18, −0.29 | −2.78, 3.7 × 10−5 | 0.98, −0.08 | −2.15, 0.04 | ||
| Hexb (U) | hexosaminidase subunit beta | 12.68, 12.48 | −1.14, 0.23 (NS) | 12.77, 12.49 | −1.20, 0.11 (NS) | ||
| Ctss | cathepsin S | 14.68, 15.04 | 1.30, 0.01 | 14.70, 15.05 | 1.29, 0.06 (NS) | ||
| C1qb | complement C1q B chain | 12.73, 13.23 | 1.44, 0.001 | 12.66, 13.36 | 1.66, 2.2 × 10−4 | ||
| C1qa | complement C1q A chain | 12.57, 12.82 | 1.21, 0.11 (NS) | 12.53, 12.89 | 1.31, 0.04 | ||
| Csf1r | colony stimulating factor 1 receptor | 13.48, 13.62 | 1.10, 0.54 (NS) | 13.42, 13.51 | 1.03, 0.85 (NS) | ||
| P2ry13 | purinergic receptor P2Y13 | 9.27, 8.11 | −2.04, 1.1 × 10−4 | 9.16, 8.53 | −1.46, 0.01 | ||
| Cx3cr1 | C-X3-C motif chemokine receptor 1 | 12.27, 11.55 | −1.50, 0.01 | 12.17, 11.73 | −1.31, 0.11 (NS) | ||
| Gpr34 | G protein-coupled receptor 34 | 11.39, 10.51 | −1.72, 0.02 | 11.43, 10.54 | −1.76, 0.004 | ||
| C1qc | complement C1q C chain | 11.01, 11.30 | 1.20, 0.15 (NS) | 10.85, 11.40 | 1.49, 0.11 (NS) | ||
| Mafb | MAF bZIP transcription factor B | 6.55, 7.31 | 2.06, 0.008 | 6.41, 6.74 | 1.27, 0.24 (NS) | ||
| Tgfbr1 (U) | transforming growth factor, beta receptor 1 | 10.46, 10.39 | −1.04, 0.80 (NS) | 10.60, 10.50 | −1.08, 0.62 (NS) | ||
| Fcgr1 | Fc fragment of IgG receptor Ia | 7.45, 7.15 | −1.17, 0.33 (NS) | 7.40, 7.23 | −1.12, 0.50 (NS) | ||
| Entpd1 | ectonucleoside triphosphate diphosphohydrolase 1 | 6.39, 5.52 | −1.74, 3.0 × 10−4 | 6.37, 5.86 | −1.42, 0.02 | ||
| Ltc4s | leukotriene C4 synthase | 8.11, 6.91 | −2.14, 1.4 × 10−5 | 8.11, 7.35 | −1.61, 8.5 × 10−4 | ||
| Csf3r | colony stimulating factor 3 receptor | 8.30, 9.58 | 2.50, 1.2 × 10−7 | 8.24, 9.53 | 2.42, 8.6 × 10−9 | ||
| Il10ra | interleukin 10 receptor subunit alpha | 8.35, 7.75 | −1.52, 2.0 × 10−4 | 8.43, 8.07 | −1.23, 0.06 (NS) | ||
| Egr1 | early growth response 1 | 8.36, 8.81 | 1.91, 0.23 (NS) | 8.30, 7.67 | −1.39, 0,45 (NS) | ||
| Siglech | sialic acid binding Ig like lectin H | ND | ND | ND | ND | ||
| Ccl2 | C-C motif chemokine ligand 2 | 5.57, 6.34 | 2.15, 0.17 (NS) | 5.66, 5.62 | 1.03, 0.97 (NS) | ||
| Lag3 | lymphocyte activating 3 | 3.84, 3.80 | 1.01, 0.98 (NS) | 3.83, 3.65 | −1.16, 0.63 (NS) | ||
| Fos | FBJ osteosarcoma oncogene | 7.21, 7.31 | 1.09, 0.92 (NS) | 8.45, 8.37 | 1.14, 0.86 (NS) | ||
| Average log2cpm | |||||||
| STR, Saline 10,839 Genes | STR, METH 10,839 Genes | PFC, Saline 10,496 Genes | PFC, METH 10,496 Genes | ||||
| 3.93 | 3.91 | 4.13 | 4.14 | ||||
| STR, Saline Top 25 genes | STR, METH Top 25 genes | PFC, METH Top 25 genes | PFC, METH Top 25 genes | ||||
| 9.34 | 9.16 | 9.38 | 9.25 | ||||