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. 2019 Oct 23;8(4):80. doi: 10.3390/biology8040080

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Co-immunoprecipitation analysis of the interaction between NIb and TaLIP. The Green fluorescent protein (GFP) was fused at the C-terminus of NIb (NIb-GFP) and transiently co-expressed with the TaLIP-HA in N. benthamiana leaves. GFP-tagged was transiently co-expressed with TaLIP-HA in N. benthamiana leaves as the control. The TaLIP-His tag (HA) was used to co-immunoprecipitate with NIb-GFP. The blots were probed with a GFP specific antibody or a HA specific antibody. IP, immunoprecipitation with special antibody. IB, immunoblot with special antibody. The sizes of the proteins in kDa are shown to the left.