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. 2019 Oct 23;8(4):80. doi: 10.3390/biology8040080

Figure 4.

Figure 4

Bimolecular fluorescence complementation (BiFC) assay of the WYMV NIb and TaLIP interactions in Nicotiana benthamiana. The pseudo-color of the Yellow fluorescent protein (YFP) signal was changed into green for convenience. The red fluorescent signal is a chloroplast auto-fluorescence signal. β-Galactosidase (GUS)-fused vectors pGTQL1211-GUS-cYFP/pGTQL1221-NIb-nYFP (YN-NIb/YC-GUS) and pGTQL1221-GUS-nYFP/pGTQL1211-TaLIP-cYFP (YN-GUS/YC-TaLIP) are the negative controls. Fluorescence photographs were taken at 3 dpi. Scale bar: 50 μm.