Table 6.

Validation of potentially altered miRNA–mRNA interactions in EGCG-treated bladder TCC cells.

Putative mRNA	mRNA Fold Change (EGCG/Ctrl)	Predicted miRNA	miRNA Fold Change (EGCG/Ctrl)	miRmap Score	TargetScan	miRDB	Seed Region of 3′UTR
ARRB1	−4.01	hsa-miR-185-3p	3.92	98.87	Yes	Yes	815–821, 1723–1729
ARRB1	−4.01	hsa-miR-3139	2.88	98.04	Yes	No
RBPMS	−4.45	hsa-miR-3176	2.17	97.23	Yes	No
MGAT5B	2.95	hsa-miR-3116	−4.65	98.69	Yes	Yes	201–207, 492–499, 537–543, 1263–1270
MGAT5B	2.95	hsa-miR-6724-5p	−3.96	99.88	Yes	No
NDUFS1	−26.61	hsa-miR-1285-3p	3.43	97.99	Yes	No
TNS1	−6.96	hsa-miR-1285-3p	3.43	98.55	Yes	No
TNS1	−6.96	hsa-miR-18a-3p	3.13	99.36	Yes	No
TNS1	−6.96	hsa-miR-31-5p	2.14	97.84	Yes	Yes	2786–2793, 4195–4202
TNS1	−6.96	hsa-miR-3176	2.17	99.13	Yes	No
TNS1	−6.96	hsa-miR-642a-5p	8.27	99.85	Yes	Yes	137–144, 3071–3077, 3157–3164, 3437–3443, 4219–4225
DLG2	−3.33	hsa-miR-1226-3p	2.28	99.63	Yes	Yes	3812–3819, 3825–3831, 3846–3853
DLG2	−3.33	hsa-miR-484	2.68	80.73	Yes	Yes	1104–1110
NMNAT2	−3.27	hsa-miR-185-3p	3.92	98.12	Yes	No
NMNAT2	−3.27	hsa-miR-93-3p	2.64	97.35	Yes	No
KMO	−33.32	hsa-miR-18a-3p	3.13	97.34	Yes	No
PPM1K	−2.04	hsa-miR-22-3p	2.04	97.03	Yes	Yes	299–306

The genes marked in bold were the miRNA–mRNA interactions validated in all three miRNA prediction databases.