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. 2019 Dec 2;11(12):642. doi: 10.3390/pharmaceutics11120642

Figure 3.

Figure 3

In vitro binding of fibrin filaments in the human whole blood thrombus by D7-TolA-Avi. Quality control of the structure of prepared whole blood thrombi was confirmed by SEM. Representative picture of thrombus prepared for binding experiments with a clear structure of fibrin filaments (A). D7-TolA-Avi was tested for the ability to target fibrin filaments of the human blood clots. APC-streptavidin was used for visualization. Samples were observed using Leica TCS SP 8 confocal microscope (excitation 633 nm, emission 645–700 nm). The representative picture illustrates the specific interaction of D7-TolA-Avi with fibrin in human blood thrombus (B). Detailed picture of fibrin in the blood thrombus. Non-homogeneous distribution of the signal is given by the observation of a thin confocal plane (C). Negative control—picture of thrombus incubated with non-specific ABDwt-TolA-Avi protein and APC-streptavidin (D), and a negative control—picture of thrombus incubated with APC-streptavidin (E).