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. 2019 Sep 30;8(4):74. doi: 10.3390/biology8040074

Figure 4.

Figure 4

Reoxygenation increases the expression of SOD3, GR, ferritin H and xCT in the hamster, but not in mouse RPTECs. Hamster and mouse RPTECs were subjected to 24 h warm anoxia, washed with PBS, and then subjected to 2 h reoxygenation in fresh culture medium. Cell protein was extracted, and the expression of SOD3, GR, ferritin H, and xCT were assessed by western blotting. Panel A depicts three of the nine performed experiments. The changes in expression of the evaluated proteins followed the changes in Nrf2 levels. Compared to hamster RPTECs not subjected to anoxia and reoxygenation, the levels of SOD3, GR, ferritin H, and xCT after the 2 h of reoxygenation increased considerably. On the contrary, the expressions of SOD3, GR, ferritin H, and xCT did not alter significantly in mouse RPTECs (B,C,D,E). An asterisk corresponds to a p < 0.001 compared to the control cells, and error bars to SEM.