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. 2019 Dec 4;11(12):653. doi: 10.3390/pharmaceutics11120653

Figure 5.

Figure 5

The effect of solvent selection on the particle size of cationic liposomes. Liposomes were manufactured as outlined in Figure 3. A flow rate ratio of 3:1 aqueous:organic phase was used with 4 mg/mL total lipid concentration in the organic phase and Tris buffer (10 mM, pH 7.4) as the aqueous phase. Particle size and PDI (columns and open circles, respectively) for (A) DOPE:DOTAP 1:1 w/w, (B) HSPC:Chol:DOTAP 1:2:4 w/w and (C) HSPC:Chol:DOTAP:DSPE-PEG2k 1:2:4:0.5 w/w. The zeta potential for these three formulations are shown in (D), (E) and (F) respectively. Results represent mean ± SD from three independent batches.