Figure 9.

Controlling the vesicle size of cationic liposomes using solvent mixtures. DOPE:DOTAP (1:1 w/w) liposomal formulations were prepared using the NanoAssemblr^TM bench-scale system. A flow rate ratio of 1:1 aqueous:organic phase was used with 4 mg/mL total lipid concentration in the organic phase and Tris buffer (10 mM, pH 7.4) as aqueous phase. Solvent mixtures of methanol/ethanol and IPA were tested. Results are shown as (A) Particle size, (B) PDI, (C) Intensity plots and (D) Zeta potential of the formulations. Results represent mean ± SD from three independent batches.