Figure 5.

Histone 2A (H2A) did not affect the number of intracellular B. besnoiti rosettes. B. besnoiti-infected BUVEC (three different isolates, N = 3) were treated with H2A (10 or 100 µg/mL) at 4 and 12 h.p.i. At 24 h.p.i. cell layers were analyzed by randomly taking five microscopic images using phase contrast microscopy. The total number of B. besnoiti rosettes per host cell was determined and the percentage of host cells carrying different numbers of PV was estimated and sub-grouped in different categories (with 1–5, 6–10 and >10 rosettes/cell, respectively; (A)). Furthermore, the infection rate (B) and the total number of tachyzoites being released into cell supernatant within 30 h.p.i. was estimated via qPCR (C). Bar graph shows mean ± SD. (n = 238).