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. 2019 Oct 11;8(4):78. doi: 10.3390/biology8040078

Figure 6.

Figure 6

Bb-NETs and A231887-NETs induced a decrease in rosette diameter in B. besnoiti-infected BUVEC. Bb-NETs or A231887-NETs were added at two different concentrations (1X and 2X) to infected BUVEC cells (three different isolates, N = 3) at 4 (A, upper panel) and 12 (A, lower panel) h.p.i. At 24 h.p.i. experimental conditions were documented within five random pictures using a phase contrast microscope and the diameter of B. besnoiti rosettes was determined (n = 1115). Box and whiskers plot, line at median, bars indicating maximum and minimum values. Statistical significance was determined by Kruskal-Wallis test followed by a Dunn´s post-test comparing experimental versus control condition at 4 and 12 h.p.i. (A). Representative images infected BUVEC cells to which Bb and A23187 NETs were added at 12 h.p.i. are shown (B). ns = non significant, ** p < 0.01, **** p < 0.0001.