Figure 7.

Effects of Bb-NET and A23187-NET treatments on intracellular B. besnoiti rosettes formation and tachyzoite release. B. besnoiti-infected BUVEC (three different isolates, N = 3), were treated with Bb-NETs or A231887-NETs at 1X and 2X concentrations at 4 and 12 h.p.i. All treatments were performed in duplicates. At 24 h.p.i. cells were analyzed by randomly taking five images for each experimental condition using phase contrast microscopy. The total number of B. besnoiti rosettes per host cell at 4 (n = 1115) and 12 h.p.i. (n = 970) was determined and the percentage of host cells carrying different numbers of rosettes was calculated (A) In addition, the total number of tachyzoites being released into cell supernatant within 30 h.p.i. was estimated via qPCR (B). Bars represents mean ± SD.