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Relative amount of acidic and basic species obtained by high-performance (HP)-weak cation exchange chromatography for trehalose-based formulation F2 at (a) 4 °C and (b) 40 °C storage temperature. In (c), the relative percentages of monomer and high molecular weight species (HMW) at the respective storage temperature over storage time gained by HP-size exclusion chromatography (SEC) analysis are presented.
