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. 2019 Nov 13;9(4):134. doi: 10.3390/bios9040134

Table A1.

Estimated apparent dissociation constants, amplitudes, and Kd confidence of the complementary oligonucleotides before optimization was carried out. Apparent Kd values are stated with “≥” since an exact determination of Kd is only possible when the binding curve reaches a plateau at high concentrations. In case the plateau is not reached, the binding curves can only be used to determine the lowest possible Kd, while the real value could be higher.

Oligonucleotide Amplitude (AU) Apparent Kd (nM) Kd Confidence (nM)
1 3.45 ≥50 ±6.57
2 11.94 ≥100 ±3.46
3 35.55 ≥660.91 ±801.61