Co-cultured Cell types
|
|
Human trophoblast (BeWo b30) cell line |
Human trophoblast (JEG-3) cell line |
Mouse oocyte |
Human primary endometrial stromal cells |
|
Human primary placental villous endothelial cells |
Human umbilical vein endothelial cells (HUVECs) |
Mouse endometrial epithelial cells |
HUVECs |
Culture Environment
|
Medium |
Dulbecco’s Modified Eagle Medium (DMEM) and Endothelial cell growth medium (EGM™-2) |
DMEM and EGM™-2 |
EmbryoMax® Modified M16 Medium |
EGM™-2 |
Temperature |
37 °C |
37 °C |
37 °C |
37 °C |
O2 tension |
Not applicable (N.A.) |
N.A. |
N.A. |
95% |
CO2
|
N.A. |
5% |
5% |
5% |
Device characteristics
|
Top Layer |
Channel: 1 mm × 1.5 cm × 135 μm (w × l × h) |
Channel: 500 μm × 200 μm (w × h) |
Zigzag Channel: 500 μm × 110 μm (w × h) |
Chamber: 7.75 mm × 6.2 mm |
Bottom Layer |
Channel: 1 mm × 1.5 cm ×135 μm (w × l × h) |
Channel: 500 μm × 200 μm (w × h) |
Channels: 6 mm × 3 mm × 0.11 mm (l × w × h) |
Chamber: 7.75 mm × 6.2 mm |
|
Device Material |
Polydimethylsiloxane (PDMS) |
PDMS |
PDMS |
PDMS |
|
Membrane Material |
Polycarbonate (PC) |
Vitrified collagen |
PC |
Epoxy resin (EPON 1002F) |
|
Membrane Pore size |
1 μm |
N.A. |
8 μm |
2 μm |
Culture characteristics
|
Coating |
Fibronectin |
Fibronectin and gelatin |
Gelatin |
Matrigel |
|
Static versus dynamic |
Dynamic |
Dynamic |
Dynamic |
Static/dynamic |
|
Flow rate |
100 μL h−1
|
30 μL h−1
|
10 μL h−1
|
1 μL min−1
|