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. 2019 Nov 20;7(12):588. doi: 10.3390/microorganisms7120588

Figure 2.

Figure 2

Gating strategy to determine CD107 positive and interferon gamma (IFNγ) positive NK cells. (A) We gated single cells based on forward scatter-area (FSC-A) and forward scatter-width (FSC-W). (B) Within the single cells, PKH26 labeled NK cells were gated for the subsequent analysis of CD107 expression (D–G) and IFNγ expression (H–K). (C) We found that CD107 was expressed on cells with a low forward scatter. (D) IFNγ plotted against FSC-A scatter showed one population of cells that expressed IFNγ. (E) Lymphocytes were gated based on forward and side scatter. (F) Within this gate, we selected the CD107 positive cells and determined GFP positive cells in (G) MDV-infected and (H) mock-infected cultures. (I) Lymphocytes were gated based on FSC-A and side scatter-area (SSC-A). (J) We then selected the IFNγ producing cells within the lymphocyte gate and subsequently determined GFP positive cells in (K) MDV-infected and (L) mock-infected cultures.