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. 2019 Dec 4;6(4):110. doi: 10.3390/bioengineering6040110

Figure 5.

Figure 5

Expression of fibrotic markers in stromal constructs and epithelial–stromal co-cultures. Expression of collagen type III (Col 3) in (A) hCF control constructs and (B) epithelial–stromal (hCEC/hCF) co-culture. High magnification of a single plane of focus in the (C) epithelial layer and (D) stromal layer show high expression of collagen type III by hCFs and little expression by hCECs. Expression of α-smooth muscle actin in the (E) epithelial layer (max projection) and (F1) stromal layer (max projection) of a co-culture show similar high expression by hCFs with low expression by hCECs. (F2) Expression of α-smooth muscle actin in hCF control. Imaged using a 40× objective lens. Scale bar = 10 μm (C and D) and 50 μm (E and F).