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. 2019 Dec 4;6(4):110. doi: 10.3390/bioengineering6040110

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Fibronectin (cFN) expression in hCF stromal constructs and epithelial–stromal co-cultures. (A) hCF construct only, showing no fibronectin staining. (B₁) Epithelial–stromal (hCEC/hCF) co-culture showing the expression of fibronectin at the epithelial–stromal interface similar to (B₂) rat cornea 1-week post-keratectomy. (C) Max projection image of the hCEC layer showing little expression in the epithelial layer. (D) Max projection image of the hCF layer showing high fibronectin expression at the epithelial–stromal interface. Imaged using a 40× objective lens.