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. 2019 Nov 26;295(2):363–374. doi: 10.1074/jbc.RA119.009788

Figure 5.

Figure 5.

FXII-W268R activity in plasma overrides C1-INH activity. FXII and HK fragmentation were analyzed by Western blotting (reduced) in a buffered system or in FXII-immunodepleted plasma in the absence or presence of PKa. A, spontaneous FXII fragmentation in buffer. B, FXII cleavage by PKa in buffer. Black arrows indicate FXIIa light chain. C and D, densitometric quantification of FXIIa light chain. The y axis reflects the band intensity of A and B, respectively. E, spontaneous FXII fragmentation in plasma. F, FXII cleavage by PKa in plasma. Open arrows indicate the FXIIa–C1-INH complex. G and H, densitometric quantification of FXIIa–C1-INH complex bands. The y axis reflects band intensity of E and F, respectively. I, spontaneous HK cleavage in plasma in the presence of FXII variants. J, PKa-triggered HK cleavage in plasma in the presence of FXII variants. K and L, densitometric quantification of HK antigen. The y axis reflects band intensity of I and J, respectively. Conditions with FXII-WT were compared with FXII-W268R and FXII-WT/W268R using two-way analysis of variance. *, p < 0.05; **, p < 0.01; ***, p < 0.001; ****, p < 0.0001.