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. 2019 Nov 19;295(2):481–503. doi: 10.1074/jbc.RA119.011341

Figure 3.

Figure 3.

Thiosemicarbazones up-regulate inhibitors of c-Met, namely NDRG1 and MIG6, but down-regulate c-Met by NDRG1- and MIG6-independent mechanism(s). DU145 or Huh7 cells were incubated with Opti-MEM media containing nontargeting, negative control siRNA (siControl) or siRNA specific for NDRG1 (siNDRG1; A–D) or MIG6 (siMIG6; E–H) in the absence of HGF and then incubated with Control medium, or this medium containing Dp44mT or DpC at 5 μm for 24 h at 37 °C. Western blot analysis was then performed. Densitometric analyses are mean ± S.E. (n = 3) normalized to the protein-loading control, β-actin. *, p < 0.05; **, p < 0.01; ***, p < 0.001 relative to the respective Control. #, p < 0.05; ##, p < 0.01; ###, p < 0.001 relative to siControl samples.