Table 1.
Equilibrium dissociation constants for the SAM-C2 peptide interactions
All peptides contained a fluorescein tag coupled to the N terminus of the peptide via a 6-aminohexanoic acid spacer.
| Peptide (Sequence) | KDa |
|---|---|
| μm | |
| TNS3-C2 peptide (CYHKKYRSATRD) | 2.18 ± 0.45 |
| 244TNS3-C2 (Cys244-Ser) (SYHKKYRSATRD) | 3.58 ± 1.09 |
| PTEN-C2 peptide (GPTRREDKFMYF) | 0.87 ± 0.18 |
| PTEN-C2-FFHK (FFHKQNKMLKKD) | 14.05 ± 1.51 |
| PTEN-C2-NRYF (NRYFSPNFKVKLY) | 25.87 ± 2.87 |
a KD values were derived from fluorescence polarization measurements.