Figure 3.

Biophysical studies of PfCSP4/38. A, distributions of hydrodynamic radii obtained from DLS on PfCSP4/38 at three different temperatures. B, CD spectra of PfCSP4/38 measured at two different temperatures: 20 and 90 °C (red dashed line). CD spectra were recorded at 20 °C before (dark solid line) and after (gray dashed line) heating the protein sample at 90 °C. Mean residue ellipticity (deg cm² dmol⁻¹) is plotted as a function of the wavelength (nm). C, differential scanning fluorimetry (nano-DSF) spectra of PfCSP4/38. Fluorescence intensities at single wavelengths of 350 nm (F₃₅₀) and 330 nm (F₃₃₀) were recorded. The F₃₅₀/F₃₃₀ ratio (upper panel) and its first derivative (lower panel) are plotted as functions-of the temperature.