| Step | Sample |
|---|---|
| 10 | Cell lysate |
| 12 | Cleared cell lysate |
| 12 | Cell pellet after clearing of lysate |
| 15 | Flow-through following StrepTactin batch-binding |
| 15 | StrepTactin resin before SUMO protease cleavage |
| 17 | Eluted fraction post SUMO protease cleavage |
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