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. Author manuscript; available in PMC: 2020 Apr 1.
Published in final edited form as: Nat Protoc. 2019 Sep 23;14(10):2986–3012. doi: 10.1038/s41596-019-0210-2

Table 2:

Example sequences for testing SHERLOCK

Name Sequence Purpose
American ZIKV synthetic RNA
Zika American synthetic RNA gacaccggaacuccacacuggaacaacaaagaagcacugguagaguucaaggacgcacaugccaaaaggcaaacugucgugguucuagggagucaagaaggagcaguucacacggcccuugcuggagcucuggaggcugagauggauggugcaaagggaaggcuguccucuggc American ZIKV synthetic RNA
Zika American strain RPA forward primer gaaatTAATACGACTCACTATAGGGCGGAACTCCACACTGGAACAACAAA RPA primer for pre-amplification of American ZIKV
Zika American strain RPA reverse primer TGCACCATCCATCTCAGCCTCCAGAGCTCC RPA primer for pre-amplification of American ZIKV
Zika American LwaCas13a crRNA gauuuagacuaccccaaaaacgaaggggacuaaaacacucccuagaaccacgacaguuugccuu American ZIKV LwaCas13 detection crRNA
Zika American LwaCas13a crRNA IVT template aaggcaaactgtcgtggttctagggagtgttttagtccccttcgtttttggggtagtctaaatcccctatagtgagtcgtattaatttc American ZIKV LwaCas13 detection crRNA IVT template
Synthetic DNA 1
Synthetic DNA 1 ggccagtgaattcgagctcggtacccggggatcctctagaaatatggattacttggtagaacagcaatctactcgacctgcaggcatgcaagcttggcgtaatcatggtcatagctgtttcctgtgtttatccgctcacaattccacacaacatacgagccggaagcataaag synthetic DNA 1
synthetic DNA 1 RPA forward primer AATTCTAATACGACTCACTATAGGGATCCTCTAGAAATATGGATTACTTGGTAGAACAG RPA primer for pre-amplification of DNA 1
synthetic DNA 1 RPA reverse primer GATAAACACAGGAAACAGCTATGACCATGATTACG RPA primer for pre-amplification of DNA 1
synthetic DNA 1 LwaCas13a crRNA gauuuagacuaccccaaaaacgaaggggacuaaaaccgccaagcuugcaugccugcaggucgag DNA 1 LwaCas13 detection crRNA
synthetic DNA 1 LwaCas13a crRNA IVT template ctcgacctgcaggcatgcaagcttggcggttttagtccccttcgtttttggggtagtctaaatcccctatagtgagtcgtattaatttc DNA 1 LwaCas13 detection crRNA IVT template
T7–3G IVT primer gaaatTAATACGACTCACTATAGGG T7 promoter annealing oligo for IVT