SHERLOCK: Nucleic acid detection with CRISPR nucleases

. Author manuscript; available in PMC: 2020 Apr 1.

Published in final edited form as: Nat Protoc. 2019 Sep 23;14(10):2986–3012. doi: 10.1038/s41596-019-0210-2

Comparison of Cas13 and Cas12 orthologs

	LwaCas13a	LbaCas13a	CcaCas13b	PsmCas13b	AsCas12a
Organism	Leptotrichia wadei	Lachnospiraceae bacterium NK4A179	Capnocytophaga canimorsus	Prevotella sp. MA2016	Acidaminococcus sp. BV3L6
Target	ssRNA	ssRNA	ssRNA	ssRNA	ssDNA/dsDNA
DR orientation	5’	5’	3’	3’	5’
Motif preference	Poly U/AU	Poly A/AC	Poly U/UA/UC	Poly A/GA	N/A
Spacer length	28	28	30	30	20
Sensitivity	~5e5 aM¹⁶	~1e9 aM³¹	~5e6 aM¹⁶	~5e8 aM¹⁶	~5e8 aM¹⁶