Component	per reaction
F primer (100μM)	0.48 μL
R primer (100μM)	0.48 μL
Rehydration Buffer, RPA kit	59 μL
LwCas13a in SB (348 ng/mL)	2 μL
crRNA (12.5 ng/mL)	0.17 μL
RNase Alert v2 (2 μM)	6.83 μL
Murine RNase Inhibitors, 40 U/μL	2.73 μL
rNTPs	8 μL
T7 RNA polymerase, 5 U/μL	2 μL
MgCl2, 1M	0.5 μL
MgAc, RPA kit	5 μL
Ultragrade water	7.82 μL
Total	95 μL