Component | Volume per reaction | Volume for four replicates |
---|---|---|
Ultragrade water | 12.32 μL | 61.6 μL |
HEPES pH 6.8, 1M | 0.4 μL | 2 μL |
MgCl2, 1M | 0.18 μL | 0.9 μL |
rNTP solution mix, 25 mM each | 0.8 μL | 4 μL |
LwaCas13 in SB (63.3 μg/mL) | 2 μL | 10 μL |
Murine RNase Inhibitors, 40 U/μL | 1 μL | 5 μL |
T7 RNA polymerase, 5 U/μL | 0.5 μL | 2.5 μL |
crRNA (10 ng/μL) | 1 μL | 5 μL |
LF-RNA sensor 1 (100 μM) | 0.2 μL | 1 μL |
Total | 19 μL | 95 μL |