Skip to main content
. Author manuscript; available in PMC: 2020 Jan 26.
Published in final edited form as: Science. 2019 Jul 11;365(6451):382–386. doi: 10.1126/science.aax7063

Figure 3: RESCUE and REPAIR multiplexing and specificity enhancement via guide engineering.

Figure 3:

A. Schematic of multiplexed C to U and A to I editing with pre-crRNA guide arrays

B. Simultaneous C to U and A to I editing on CTNNB1 transcripts

C. Schematic of rational engineering with guanine base flips to prevent off-target activity at neighboring adenosine sites.

D. Percent editing at on-target C and off-target A sites for Gaussia luciferase (left) and KRAS (right) using rational introduction of disfavored base flips.