FIGURE 9:

The all-β-sheet conformation of XCL1 exhibits significant amphipathic character similar to the α-defensin Defa4. A: Structure of mouse Defa4 (PDB ID 1TV0) with electrostatic surfaces mapped to highlight amphipathic character. B: Human XCL1 dimeric structure (PDB ID 2JP1) showing electrostatic potential across the surface. C: Individual subunit of human XCL1 mapping electrostatic potential demonstrating that one half of the subunit has a large patch of positive charge while the other half, usually obscured by the second subunit, has a large hydrophobic patch resulting in an amphipathic molecule