Skip to main content
. 2020 Jan 6;10:2998. doi: 10.3389/fimmu.2019.02998

Figure 1.

Figure 1

Asthmatic bronchial smooth muscle cell co-culture increases rhinovirus-mediated monocyte migration. (A) Monocyte migration was assessed in response to supernatants of reconstituted bronchial epithelial cells in air liquid interface infected or not with human rhinovirus (HRV-16; at MOI 0.1 for 1 h). The effect of CCL2 on rhinovirus-induced monocyte migration was evaluated by addition of blocking antibody (n = 7–11 per group). (B) CCL2 proteins were assessed from epithelial cell supernatant (n = 5 per group). (C) Monocyte migration was assessed in response to supernatants of reconstituted bronchial epithelial cells in air liquid interface co-cultured for 1 week with bronchial smooth muscle cells from control (n = 5–9 per group) or (D) asthmatic patients (n = 5–8 per group). Data are presented as mean ± SEM values of three independent experiments (Wilcoxon test, #P < 0.05; ##P < 0.01; ###P < 0.001 and ordinary one way anova, Bonferroni's multiple comparisons test, *P < 0.05 compare the mean of HRV+ alone with the mean of every other columns).