Figure 4.

Increase rhinovirus-mediated monocyte migration induced by asthmatic bronchial smooth muscle is CCL5 dependent. Monocyte migration was assessed in response to recombinant CCL5 (CCL5 Rec) (A) as a positive control, or to supernatants of reconstituted bronchial epithelial cells in air liquid interface co-cultured with bronchial smooth muscle cells from (B) control (n = 6–9 per group) or (C) asthmatic patients (n = 6–8 per group). The effect of CCL5 on rhinovirus-induced monocyte migration was evaluated by addition of blocking antibody (Anti-CCL5 Ab). Data are presented as mean ± SEM values of three independent experiments (Wilcoxon test, ^#P < 0.05; ^##P < 0.01 and ordinary one way anova, Bonferroni's multiple comparisons test, *P < 0.05 compare the mean of HRV+ alone with the mean of every other columns).