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. Author manuscript; available in PMC: 2021 Jan 1.
Published in final edited form as: FASEB J. 2019 Nov 28;34(1):930–944. doi: 10.1096/fj.201902155R

Figure 6. High glucose induced glycolytic enzymes and fibrogenesis.

Figure 6.

Pulmonary artery adventitial fibroblasts were starved overnight in glucose-free media followed by stimulation with normal (5 mM) or high (25 and 50 mM) glucose media for 24 hours and 72 hours. Representative Western blot images of fibroblast protein extracts showing hexokinase 2 (HK2; 100 kDa), 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase isoform 3 (PFKFB3; 60 kDa), transglutaminase 2 (TG2; 78 kDa), fibronectin (Fn; 220 kDa), type1 collagen (Col1; 147 kDa), α-smooth muscle actin (α-SMA; 42 kDa) and β-actin (42 kDa) levels. Bar graph demonstrating changes in HK2, PFKFB3, TG2, Fn, Col1 and α-SMA expression normalized to β-actin levels by densitometry analysis. n=4–6 replicates/ treatment group; mean±SEM. Statistical analysis was performed by t-test. p<0.05 *compared to normal glucose at respective time-points.