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. Author manuscript; available in PMC: 2020 Jan 13.
Published in final edited form as: Cell Rep. 2019 Dec 17;29(12):3885–3901.e5. doi: 10.1016/j.celrep.2019.11.058

Figure 3. AAV9-STAS Rescues Vulnerable SMA Motor Neurons from Degeneration.

Figure 3.

(A) ChAT immunostaining of L1 spinal cords from SMA mice injected with AAV9-GFP, AAV9-STAS, or AAV9-SMN as well as uninjected WT mice at P11. Scale bar: 50 μm.

(B) Total number of L1 motor neurons in the same groups as in (A) at P11. Data represent means and SEM from WT (n = 4), SMA+AAV9-GFP (n = 6), SMA+AAV9-STAS (n = 7), and SMA+AAV9-SMN (n = 6). Statistics were performed with one-way ANOVA with Tukey’s post hoc test. *p < 0.05, **p < 0.01, ***p < 0.001.

(C) ChAT immunostaining of L5 spinal cords from the same groups as in (A) at P11. L5 MMC motor neurons are indicated by dotted circles and magnified in the insets. Scale bar: 125 mm.

(D) Total number of L5 MMC motor neurons in the same groups as in (A) at P11. Data represent means and SEM from WT (n = 5), SMA+AAV9-GFP (n = 5), SMA+AAV9-STAS (n = 7), and SMA+AAV9-SMN (n = 6). Statistics were performed with one-way ANOVA with Tukey’s post hoc test. **p < 0.01, ***p < 0.001.