KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| SMN (clone 8) | BD Transd Lab | Cat#610646; RRID:AB_397973 |
| GFP | Sigma | Cat#G1544; RRID:AB_439690 |
| p62 | MBL | Cat#PM045; RRID:AB_1279301 |
| LC3B | Novus | Cat#NB600-1384; RRID:AB_669581 |
| Actin | Proteintech | Cat#66009; RRID:AB_2687938 |
| Gapdh | Millipore | Cat#MAB374; RRID:AB_2107445 |
| Tubulin (DM1A) | Sigma | Cat#T9026 RRID:AB_477593 |
| p38 MAPK | Cell Signaling | Cat#8690; RRID:AB_10999090 |
| p-p38 MAPK | Cell Signaling | Cat#4511; RRID:AB_2139682 |
| p53 | Leica Novocastra | Cat#NCL-p53-CM5p; RRID:AB_563933 |
| p-p53S15 | Cell Signaling | Cat#9284 (Lots: 12, 15) |
| Parvalbumin | Covance | custom made |
| VGluT1 | Covance | custom made |
| Synaptophysin | Synaptic Systems | Cat#101-004; RRID:AB_1210382 |
| NF-M | Millipore | Cat#AB1987; RRID:AB_91201 |
| ChAT | Millipore | Cat#AB144; RRID:AB_90650 |
| Bungarotoxin | Invitrogen | Cat#T1175; RRID:AB_2313931 |
| DAPI | Molecular Probes | Cat#D3571; RRID:AB_2307445 |
| 488 Anti-rabbit | Jackson | 711-545-152; RRID:AB_2313584 |
| 488 Anti-goat | Jackson | 705-545-147; RRID:AB_2336933 |
| 488 Anti-chicken | Jackson | 703-545-155; RRID:AB_2340375 |
| Cy3 Anti-rabbit | Jackson | 711-165-152; RRID:AB_2307443 |
| Cy3 Anti-mouse | Jackson | 715-165-150; RRID:AB_2340813 |
| Cy3 Anti-goat | Jackson | 705-165-147; RRID:AB_2307351 |
| Cy5 Anti-goat | Jackson | 705-175-147; RRID:AB_2340415 |
| Cy5 Anti-guinea pig | Jackson | 706-175-148; RRID:AB_2340462 |
| HRP Anti-mouse | Jackson | 115-035-044; RRID:AB_2338503 |
| HRP Anti-rabbit | Jackson | 111-035-003; RRID:AB_2313567 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Puromycin dihydrochloride | Sigma | P8833 |
| Bafilomycin A1 | Alfa Aesar | J67193XF |
| MW150 | Roy et al., 2019 | CAS 1628502-91-9 |
| Critical Commercial Assays | ||
| ViraPower Lentiviral Packaging Mix | Invitrogen | K497500 |
| RevertAid RT Reverse Transcription Kit | Thermo Fisher Scientific | Cat#K1691 |
| Experimental Models: Cell Lines | ||
| Mouse: NIH 3T3-StasRNAi | This paper | N/A |
| Experimental Models: Organisms/Strains | ||
| Mouse: FVB.Cg-Tg(SMN2*delta7)4299Ahmb Tg(SMN2)89Ahmb Smn1tm1Msd/J | The Jackson Laboratory | JAX:005025; RRID:IMSR_JAX:005025 |
| Mouse: B6;129S6-Chattm2(cre)Lowl/J Mus musculus | The Jackson Laboratory | JAX:006410; RRID:IMSR_JAX:006410 |
| Mouse: B6;129P2-Pvalbtm1(cre)Arbr/J | The Jackson Laboratory | JAX:008069; RRID:IMSR_JAX:008069 |
| Mouse:Grm7Tg(SMN2)89Ahmb Smn1tm3(SMN2/Smn1)Mrph Tg(SMN2*delta7) 4299Ahmb/J | The Jackson Laboratory | JAX:007951; RRID:IMSR_JAX:007951 |
| Oligonucleotides | ||
| RT-qPCR primers for Stas mRNA, see Table S1 | Lotti et al., 2012 | N/A |
| RT-qPCR primers for Stas Aber mRNA, see Table S1 | Lotti et al., 2012 | N/A |
| RT-qPCR primers for H1c pre-mRNA, see Table S1 | Tisdale et al., 2013 | N/A |
| RT-qPCR primers for Cdkn1a mRNA, see Table S1 | Ruggiu et al., 2012 | N/A |
| RT-qPCR primers for Gapdh mRNA, see Table S1 | Ruggiu et al., 2012 | N/A |
| RT-qPCR primers for SMN2 FL mRNA, see Table S1 | Ruggiu et al., 2012 | N/A |
| RT-qPCR primers for Chodl mRNA, see Table S1 | Van Alstyne et al., 2018a | N/A |
| RT-qPCR primers for STAS mRNA, see Table S1 | This paper | N/A |
| RT-qPCR primers for GFP mRNA, see Table S1 | This paper | N/A |
| RT-PCR primers for Mdm2 mRNA (E2-5), see Table S1 | Van Alstyne et al., 2018a | N/A |
| RT-PCR primers for Mdm4 mRNA (E5-8), see Table S1 | Van Alstyne et al., 2018a | N/A |
| PCR primers for genotyping mouse Smn, see Table S1 | Simon et al., 2017 | N/A |
| PCR primers for genotyping ChAT-Cre, see Table S1 | Fletcher et al., 2017 | N/A |
| PCR primers for genotyping PV-Cre, see Table S1 | Fletcher et al., 2017 | N/A |
| qPCR primers for AAV titration, see Table S1 | This paper | N/A |
| Antisense morpholino oligonucleotides, see Table S1 | Gene Tools | N/A |
| Stasimon shRNA targeting sequence: GGAAGACCCGTTGTATACA | This paper | N/A |
| Recombinant DNA | ||
| dsAAV-CB-GFP | This paper | N/A |
| dsAAV-CB-STAS | This paper | N/A |
| dsAAV-CB-SMN | This paper | N/A |
| p.Lenti.puro/StasRNAi | This paper | N/A |
| pSUPERIOR.puro | OligoEngine | Cat#VEC-IND-0006 |
| pRRL.SIN.cPPT.PGK-GFP.WPRE | Addgene | Plasmid 12252 |
| Software and Algorithms | ||
| LAS X | Leica | N/A |
| Prism 6 | GraphPad | N/A |