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. 2020 Jan 8;13:185–197. doi: 10.2147/OTT.S229577

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© 2020 Huang et al.

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Expression of UBE4B protein in RCC cell lines by Western blotting. (A) Representative Western blotting of UBE4B protein expression in five human RCC cell lines. (B) Expression of UBE4B protein in human RCC cell lines was upregulated in ACHN, A498, 786-O, CaKi-1 and SKRC39 cells. Relative protein levels of UBE4B in different cell lines were shown as mean ± SD. (C, D) Among the four UBE4B-targeted small interfering RNAs (siUBE4B), siUBE4B#2 and siUBE4B#3 showed higher knockdown efficiencies after 48 hrs’ transfection. siNC represents negative control small interfering RNAs.